studies on the binding of dna with the inclusion of brilliant green inside the cavity of γ-cyclodextrin

the interaction of brilliant green with herring sperm dna was investigated in detail by spectrometric methods in γ-cyclodextrin systems. on the condition of physiological ph, brilliant green prefers to form the 1:1 inclusion complex with γ-cyclodextrin.all the evidences indicated that the binding modes between γ-cyclodextrin-brilliant green and dna were grooving binding and partial non-classical intercalative binding. the binding ratio of the inclusion complex with dna is 6:1. the calculated thermodynamic parameters suggested that the binding of the inclusion complex to dna was driven mainly by entropy.